Megan Campbell will miss the Republic of Ireland's must-win European qualifier against Germany at Tallaght Stadium.
The Manchester City defender had been engaged with a return to play protocol with her club and the Ireland coaching and medical team were in regular contact with Manchester City.
The 27-year-old picked up a knock in training on Wednesday and will return to City for further attention.
Birmingham City captain Harriet Scott will link up with Vera Pauw's squad on Friday, following on from Louise Quinn who arrived into camp on Tuesday.
The squad have returned negative results following their latest round of testing for COVID-19.
All players and staff members were tested prior to meeting up in Dublin on Sunday and then tested again when in camp.
The Girls in Green need a win next Tuesday to guarantee second place in the group but they lost 3-0 away to Germany earlier in the campaign.
Republic of Ireland WNT Squad
Goalkeepers: Marie Hourihan (SC Braga), Courtney Brosnan (West Ham United), Grace Moloney (Reading), Niamh Reid-Burke (Peamount United)
Defenders: Harriet Scott (Birmingham City), Keeva Keenan (Celtic), Claire Walsh (Peamount United), Louise Quinn (Fiorentina), Diane Caldwell (SC Sand), Claire O'Riordan (MSV Duisburg), Eabha O'Mahony (Cork City), Isibeal Atkinson (Shelbourne), Aine O'Gorman (Peamount United)
Midfielders: Niamh Fahey (Liverpool), Jamie Finn (Shelbourne), Denise O'Sullivan (Brighton & Hove Albion - on loan from NC Courage), Niamh Farrelly (Peamount United), Ruesha Littlejohn (Leicester City), Ellen Molloy (Wexford Youths), Jessica Ziu (Shelbourne), Emily Whelan (Shelbourne)
Forwards: Katie McCabe (Arsenal), Heather Payne (Florida State University), Leanne Kiernan (West Ham United), Amber Barrett (FC Koln), Rianna Jarrett (Brighton & Hove Albion)