Taoiseach Micheál Martin has said that the employees being laid off by Twitter should "be treated with respect".
The company began layoffs among its 7,500 workforce on Friday and some of its 500 Irish employees are being let go now.
New Twitter CEO Elon Musk has received backlash for his handling of the changeover, with many employees finding themselves suddenly locked out of buildings and email accounts before being informed they had been laid off.
Taking to the social media platform, Mr Musk tweeted that he had "no choice" but to reduce the workforce, while the company is "losing over $4 million per day".
In another tweet he said that the loss of revenue was "due to activist groups pressuring advertisers, even though nothing has changed with content moderation".
Taoiseach Micheál Martin told the Irish Independent that the quick changes are "unacceptable".
"There has to be respect - there has to be dignity in situations like this", he said.
"We are concerned."
"The summary notice of dismissal or you are no longer working (for Twitter), is not acceptable.
"That is not the way we conduct industrial relations or organise relationships between employers and employees."
Regarding Twitter’s reduction in force, unfortunately there is no choice when the company is losing over $4M/day.
Everyone exited was offered 3 months of severance, which is 50% more than legally required.
— Elon Musk (@elonmusk) November 4, 2022
So far, around half of Twitter's workforce has been laid off under the company's new owner.
Simon Balmain learnt he was losing his job in the UK on Thursday night and told Newstalk that it's been an emotional rollercoaster for staff.
"It really has been difficult for people to really stay focused. not knowing what's going to happen", he said.
"I personally try to keep my head down and focus on the work, but it's definitely been incredibly distracting over the last few months."