Tennis star Novak Djokovic’s visa has been cancelled by the Australian Government.

Australian Immigration Minister Alex Hawke took the decision just days before the World Number One was due to begin his defence of his Australian Open title.

He exercised is personal power to cancel the visa after Australian Prime Minister Scott Morrison said he expected authorities to apply Government policy which requires non-nationals to be either double vaccinated or provide an acceptable medical exemption to enter the country.

It comes just days after the Serbian star won his appeal against the cancellation of his visa, with the Australian Federal Court releasing him from hotel quarantine.

Australian Immigration Minister Alex Hawke statement on Novak Djokovic.

"Today I exercised my power under section 133C(3) of the Migration Act to cancel the visa held by Mr Novak Djokovic on health and good order grounds, on the basis that it was in the public interest to do so," Minister Hawke said in a statement.

He said the decision was taken “on the basis that it was in the public interest to do so”.

“The Morrison Government is firmly committed to protecting Australia’s border, particularly in relation to the COVID-19 pandemic,” he said.

“I thank the officers of the Department of Home Affairs and the Australian Border Force who work every day to serve Australia’s interests in increasingly challenging operational environments.”

The Australian Prime Minister Scott Morrison speaking at the COP26 UN Climate Summit. Image: Iain Masterton/Alamy Live News

Djokovic was originally detained on his arrival in Melbourne after his medical exemption visa was cancelled on his arrival in the country.

Authorities decided the tennis star who is outspokenly opposed to getting vaccinated, had not provided sufficient medical evidence to qualify for the exemption.

Court documents alter showed that he had been granted the visa on the basis that he had picked up the virus in December.

He won his appeal against the decision on Monday and was released from the State-run quarantine hotel where he was being detained.

The Australian Government made it clear at the time that Minister Hawke retained the power to cancel Djokovic’s visa – a move that would reportedly bar him from the country for three years.

Minister Hawke has now exercised that power.

Djokovic’s legal team are now likely to file a court injunction appealing the decision.