The Green Party's Grace O'Sullivan and Fine Gael's Deirdre Clune have both been elected as MEPs for Ireland South.
Both were deemed elected after the final count, without reaching the quota.
Senator O'Sullivan took the fourth seat - meaning she will immediately be able to take her seat once the new European Parliament term starts.
Ms Clune, who is a sitting MEP, will have to wait until Britain leaves the EU to take the fifth seat in the constituency.
Following the 20th and final count, Grace O'Sullivan had 119,701 votes to Deirdre Clune's 112,162.
Ireland's final two MEPs were elected in Cork following the distribution of the surplus from Independents 4 Change candidate Mick Wallace.
Mr Wallace took the third seat earlier this afternoon.
Fine Gael's Sean Kelly and Fianna Fáil's Billy Kelleher had previously been deemed elected in the constituency.
Ireland South was the final European constituency to finish counting, after a full recount was ordered last week.
The recount got underway yesterday, but ended after Sinn Féin's Liadh Ní Riada conceded.
With the final results now in, Ireland's 13 MEPs have been decided - five from Fine Gael; two from both Fianna Fáil and the Green Party; two representing the Independents 4 Change grouping; one from Sinn Féin; and one independent.
As well as Deirdre Clune, Fianna Fáil's Barry Andrews in Dublin was elected to the other so-called 'Brexit' seat - meaning he'll also have to wait to take his seat.