A forensics team says DNA found on the garden hoe used in the killing of two British backpackers in Thailand does not match that of the two defendants.
The bodies of Hannah Witheridge and David Miller were found on a beach on the holiday island of Koh Tao last September.
Prosecutors say Miss Witheridge, 23, from Norfolk, had been raped and beaten. Mr Miller, 24, from Jersey, drowned in the sea after being clubbed around the head with the hoe.
Burmese bar workers Zaw Lin and Wei Phyo, both 22, have pleaded not guilty.
The trial has previously heard claims the pair were tortured and threatened with death if they did not confess.
Both men say they eventually confessed after being told they would only go to prison for four or five years.