Around three out of five - or 57% - of Dublin companies say traffic congestion is having an increasing impact on their business.
The Dublin Chamber of Commerce asked firms whether the negative impact of congestion had increased over the past year. 57% of respondents said the indirect costs of congestion had increased, while 41% noted no difference.
The survey was carried out in the first two weeks of November, and answered by 303 respondents.
The findings indicate many Dublin businesses are having to change the way they operate to combat the effects of congestion.
A number of companies also indicated that they have had to become much more flexible with employees in terms of starting times.
A significant number say they are allowing staff to work more from home - while others said that they have had to increase technology spend to allow people to work remotely.
And more drastic action is also being taken, with one firm admitting to having moved their business from the city centre to outside the M50.
Another company said they had moved from the city centre to a business park in the suburbs - which has resulted in staff members reducing commute times by one hour per day.
Dublin Chamber CEO Gina Quin said: "The results of the survey show that significant under-investment in transport infrastructure over recent years is now coming back to bite us".
"Our transport system is considerably behind where it needs to be. We are not spending enough on new transport infrastructure. Currently we're spending €150m per annum, but we need to triple that investment to bring our per capita spend into line with cities like Manchester and London - cities we are competing with for FDI and jobs".
Dublin Chamber has warned that the combination of continued economic growth and an ever-increasing population means that congestion in the city is only going to get worse.
The Chamber has also urged the Government and the National Transport Authority to find a way to deliver Metro North quicker than the proposed finish date of 2026.
