Police and enforcement officers have also launched an investigation
A nightclub has been shut down after video emerged showing a scantily clad woman riding a horse inside the packed venue, before it appears to panic and throws her off.
Clubbers are heard gasping and screaming amid the chaos at the Florida club.
WATCH: Mokai Lounge in Miami Beach under fire after video surfaced of a horse inside the nightclub pic.twitter.com/C7Saz3q20a— Joel Franco (@OfficialJoelF) March 9, 2018
Miami Beach city manager Jimmy Morales said he had revoked Mokai Lounge's business licence.
Police and enforcement officers have also launched an investigation after receiving multiple complaints about the incident at the South Beach venue on Thursday.
No injuries to humans or the animal have been reported.
The venue could appeal against the decision, but so far it has not commented.
The incident has sparked outrage online - with many concerned about the animal's welfare.
A petition to revoke the club's licence was set up and pulled in more than 1,000 signatures within hours.
"People go to nightclubs to have a good time, enjoy drinks with friends, dance and party. This environment is by no means a place for ANY animal or wildlife," the petition read.
The Miami Beach Police Department said it was "very concerned".
It later tweeted: "Miami Beach PD detectives along with @MiamiDadePD's animal experts have located the horse that was at Mokai. The horse has been inspected and deemed to be healthy and safe by Miami Dade PD."
UPDATE: Miami Beach PD detectives along with @MiamiDadePD ’s animal experts have located the horse that was at Mokai. The horse has been inspected and deemed to be healthy and safe by Miami Dade PD.— Miami Beach Police (@MiamiBeachPD) March 10, 2018
Our investigation continues. https://t.co/pCI5TZ9sww
In a statement on Friday, the City of Miami Beach said it was "made aware of an incident involving possible animal cruelty and a hazard to public safety".
Mr Morales said he was "disgusted and offended that any legitimate businessperson would think this was an appropriate action to take".
He added: "This activity was not permitted, and as soon as we became aware, I immediately instructed staff to act swiftly in remedying this situation."
Miami Beach mayor Dan Gelber said he "fully supported" the city manager's decision to revoke the club's business licence and added: "Animal cruelty is an abhorrent and vile act."