A ban on the vehicles has also been implemented in parts of Barcelona
Prague has joined Barcelona by introducing a ban on 'Segways' for parts of the city.
A number of different companies in the Czech city offer tours using the electric vehicles.
Reuters reports the ban - which applies in the city's historic centre - is in response to complaints from residents.
Prague's mayor Adriana Krnacova said: "We received countless complaints and we decided to satisfy both residents and even some tourists".
The ban will apply to pavements, streets and cycle lanes. A fine of 2,000 crowns (around €74) will apply for Segway users when the ban is implemented next month.
Earlier this month, Barcelona officials also confirmed a temporary ban on 'personal mobility vehicles' along its popular tourist waterfront.
TheLocal.es reports the 'emergency ban' applies in parts of Spain's second largest city until the end of the summer season on October 1st.
It follows complaints from residents of the Ciutat Vella district. Officials are also reported to be considering city-wide regulations.
Anybody found to be breaching the temporary ban in the Spanish city faces a €90 on-the-spot fine - and could face a penalty of up to €1,125 if caught driving in a 'dangerous' manner.