Details of owners will be stored on one of four databases
All dogs will have to be legally micro-chipped from the end of this month.
The acting Agriculture Minister Simon Coveney is reminding all dog owners of the new arrangements.
This will see dogs being micro-chipped and registered on an authorised database.
There are currently four authorised databases where details will be stored: Animark, Fido, The Irish Coursing Club and the Irish Kennel Club.
All of these will issue an ownership certificate, which allows owners to ensure registered details relating to their dog are correct.
The certificate is also needed for transfer of ownership.
The dog micro-chipping programme has been rolled out on a phased basis, having been started last year.
"It is important that dog owners understand it applies to all dogs from March 2016. Widespread micro-chipping of all dogs brings a range of benefits - including the protection of the welfare of dogs and assist with speedy identification of stray dogs and their owners", Mr Coveney said.
While many owners have already had their dogs' micro-chipped, particularly since the laws were signed last year, others are being reminded of the importance of making sure all dogs are compliant with the requirements.