The Clinton campaign responded by suggesting Mr Trump "has a pattern of inciting people to violence"
Republican US presidential candidate Donald Trump is facing criticism after being accused of hinting at the assassination of his Democratic rival Hillary Clinton for a second time.
Speaking at a rally in Miami, Mr Trump called for Mrs Clinton's bodyguards to give up their guns and "see what happens to her".
He told supporters his rival wanted to "destroy the second amendment" - referring to the right to own guns.
"I think that her bodyguards should drop all weapons, they should disarm," he told the crowd. "I think they should disarm immediately.
"Take their guns away, she doesn't want guns. Let's see what happens to her. It would be very dangerous," he added.
Mr Trump spoke against a backdrop reading 'Les Deplorables' - a reference to his rival's recent controversial comments about some of his supporters.
In a statement quoted by The Guardian, Hillary Clinton's campaign manager Robby Mook responded to Mr Trump's latest remarks, saying the Republican candidate "has a pattern of inciting people to violence".
"Whether this is done to provoke protesters at a rally or casually or even as a joke, it is an unacceptable quality in anyone seeking the job of Commander in Chief," he suggested.
Last week, Mr Trump suggested that his rival is "so protected" that "she could walk into this arena right now and shoot somebody with 20,000 people watching, right smack in the middle of the heart, and she wouldn't be prosecuted".
The latest Trump controversy comes after he finally accepted that Barack Obama was born in the US.
The Republican presidential candidate had questioned the validity of Mr Obama's birth certificate for years.